Lentiviral Vectors

Plasmid List

Packaging Plasmids

Plasmid Name Description Map Sequence Data sheet
pCAG-HIVgp CAG promoter, HIV-1 gag and pol [PDF] [Word] [Data sheet]
pCAG-HIVgp IN-D64V CAG promoter, HIV-1 gag and pol, integrase mutant [PDF] [Word] [Data sheet]
pCAG-HIVgp IN-D64E CAG promoter, HIV-1 gag and pol, integrase mutant [PDF] [Word] [Data sheet]
pCMV-VSV-G-RSV-Rev CMV promoter, VSV-G, RSV promoter, Rev [PDF] [Word] [Data sheet]
pCMV-VSV-G CMV promoter, VSV-G [PDF] [Word] [Data sheet]
pRSV-Rev RSV promoter, Rev n.a. [Word] [Data sheet]

Vector Plasmids

Fluorescent protein gene expression
Plasmid Name Description Map Sequence Data sheet
CS-CDF-CG-PRE CMV promoter, EGFP [PDF] [Word] [Data sheet]
CS-CDF-EG-PRE EF-1α promoter, EGFP [PDF] [Word] [Data sheet]
CS-CDF-UbCG-PRE UbC promoter, EGFP [PDF] [Word] [Data sheet]
CS-CA-GFP CAG promoter, EGFP [PDF] [Word] [Data sheet]
CSII-CMV-Venus CMV promoter, Venus [PDF] [Word] [Data sheet]
CSII-EF-Venus EF-1α promoter, Venus [PDF] [Word] [Data sheet]
CS-CA-Venus CAG promoter, Venus [PDF] [Word] [Data sheet]
CSII-CMV-Venus-NLS CMV promoter, Venus + nuclear localization signal [PDF] [Word] [Data sheet]
CSII-EF-Venus-NLS EF-1α promoter, Venus + nuclear localization signal [PDF] [Word] [Data sheet]
CSII-CMV-mRFP1 CMV promoter, mRFP1 [PDF] [Word] [Data sheet]
CSII-EF-mRFP1 EF-1α promoter, mRFP1 [PDF] [Word] [Data sheet]
CSII-CMV-Venus-Mito-IRES2-Bsd CMV promoter, Venus + mitochondrial targeting sequence, IRES, Blasticidin resistance [PDF] [Word] [Data sheet]
cDNA expression
Plasmid Name Description Map Sequence Data sheet
CSII-CMV-MCS CMV promoter, Multiple cloning site [PDF] [Word] [Data sheet]
CSII-EF-MCS EF-1α promoter, Multiple cloning site [PDF] [Word] [Data sheet]
CS-CA-MCS CAG promoter, Multiple cloning site [PDF] [Word] [Data sheet]
CSII-CMV-MCS-IRES2-Bsd CMV promoter, Multiple cloning site, IRES, Blasticidin resistance [PDF] [Word] [Data sheet]
CSII-CMV-MCS-IRES2-Venus CMV promoter, Multiple cloning site, IRES, Venus [PDF] [Word] [Data sheet]
CSII-EF-MCS-IRES2-Venus EF-1α promoter, Multiple cloning site, IRES, Venus [PDF] [Word] [Data sheet]
CS-UbC-MCS-IRES2-Venus UbC promoter, Multiple cloning site, IRES, Venus [PDF] [Word]
CSIV-CMV-MCS-IRES2-Venus CMV promoter, Multiple cloning site, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSIV-UbC-MCS-IRES2-Venus UbC promoter, Multiple cloning site, IRES, Venus, loxP [PDF] [Word]
CSII-CMV-MCS-IRES2-mRFP1 CMV promoter, Multiple cloning site, IRES, mRFP1 [PDF] [Word]
CSII-EF-MCS-IRES2-hKO1 EF-1α promoter, Multiple cloning site, IRES, hKO1 [PDF] [Word]
CSII-CMV-RfA CMV promoter, Gateway [PDF] [Word] [Data sheet]
CSII-EF-RfA EF-1α promoter, Gateway [PDF] [Word] [Data sheet]
CSII-CMV-RfA-IRES2-Venus CMV promoter, Gateway, IRES, Venus [PDF] [Word] [Data sheet]
CSII-EF-RfA-IRES2-Venus EF-1α promoter, Gateway, IRES, Venus [PDF] [Word] [Data sheet]
CS-UbC-RfA-IRES2-Venus UbC promoter, Gateway, IRES, Venus [PDF] [Word]
CS-CA-RfA-IRES2-Venus CAG promoter, Gateway, IRES, Venus [PDF] [Word] [Data sheet]
CSII-CMV-RfA-IRES2-hKO1 CMV promoter, Gateway, IRES, hKO1 [PDF] [Word]
CS-UbC-RfA-IRES2-hKO1 UbC promoter, Gateway, IRES, hKO1 [PDF] [Word]
shRNA expression
Plasmid Name Description Map Sequence Data sheet
pENTR4-H1 Gateway entry vector, Human H1 promoter [PDF] [Word] [Data sheet]
CS-RfA-CG Gateway, CMV promoter, EGFP [PDF] [Word] [Data sheet]
CS-RfA-EG Gateway, EF-1α promoter, EGFP [PDF] [Word] [Data sheet]
CS-RfA-CMV-mRFP1 Gateway, CMV promoter, mRFP1 [PDF] [Word] [Data sheet]
CS-RfA-EF-mRFP1 Gateway, EF-1α promoter, mRFP1 [PDF] [Word] [Data sheet]
CS-RfA-EVBsd Gateway, EF-1α promoter, Venus-2A-Blasticidin [PDF] [Word] [Data sheet]
CS-CDF-CG-PRE-RfA CMV promoter, EGFP, Gateway in the U3 region [PDF] [Word] [Data sheet]
CS-CDF-EG-PRE-RfA EF-1α promoter, EGFP, Gateway in the U3 region [PDF] [Word] [Data sheet]
CSII-CMV-mRFP1-RfA CMV promoter, mRFP1, Gateway in the U3 region [PDF] [Word] [Data sheet]
CSII-EF-mRFP1-RfA EF-1α promoter, mRFP1, Gateway in the U3 region [PDF] [Word] [Data sheet]
Tet-inducible shRNA expression
Plasmid Name Description Map Sequence Data sheet
pENTR4-H1tetOx1 Gateway entry vector, Human H1 promoter, tet operator [PDF] [Word] [Data sheet]
CS-RfA-ETBsd Gateway, EF-1α promoter, TetR-2A-Blasticidin [PDF] [Word]
CS-RfA-ETV Gateway, EF-1α promoter, TetR-2A-Venus [PDF] [Word]
CS-RfA-ETR Gateway, EF-1α promoter, TetR-2A-mRFP1 [PDF] [Word]
Tet-inducible cDNA expression
Plasmid Name Description Map Sequence Data sheet
CSIV-TRE-RfA-CMV-KT Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-RfA-UbC-KT Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
Tet-inducible GFP expression
Plasmid Name Description Map Sequence Data sheet
CSIV-TRE-GFP-CMV-KT Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-GFP-UbC-KT Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
For generating iPS cells (Human)
Plasmid Name Description Map Sequence Data sheet
CSIV-CMV-hOct3/4-IRES2-Venus CMV promoter, hOct3/4, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSIV-CMV-hSox2-IRES2-Venus CMV promoter, hSox2, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSIV-CMV-hKlf4-IRES2-Venus CMV promoter, hKlf4, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSIV-CMV-hc-Myc-IRES2-Venus CMV promoter, hc-Myc, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSIV-CMV-hLin28-IRES2-Venus CMV promoter, hLin28, IRES, Venus, loxP [PDF] [Word]
CSIV-CMV-SV40LT-IRES2-Venus CMV promoter, SV40 LT, IRES, Venus, loxP [PDF] [Word] [Data sheet]
CSII-EF-hOct3/4-IRES2-Venus EF-1α promoter, hOct3/4, IRES, Venus [PDF] [Word] [Data sheet]
CSII-EF-hSox2-IRES2-Venus EF-1α promoter, hSox2, IRES, Venus [PDF] [Word] [Data sheet]
CSII-EF-hKlf4-IRES2-Venus EF-1α promoter, hKlf4, IRES, Venus [PDF] [Word] [Data sheet]
CSII-EF-hc-Myc-IRES2-Venus EF-1α promoter, hc-Myc, IRES, Venus [PDF] [Word] [Data sheet]
CS-UbC-hOct3/4-IRES2-Venus UbC promoter, hOct3/4, IRES, Venus [PDF] [Word]
CS-UbC-hSox2-IRES2-Venus UbC promoter, hSox2, IRES, Venus [PDF] [Word]
CS-UbC-hKlf4-IRES2-Venus UbC promoter, hKlf4, IRES, Venus [PDF] [Word]
CS-UbC-hc-Myc-IRES2-Venus UbC promoter, hc-Myc, IRES, Venus [PDF] [Word]
CSIV-TRE-hOct3/4-CMV-KT Tet-responsive promoter, hOct3/4, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-hSox2-CMV-KT Tet-responsive promoter, hSox2, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-hKlf4-CMV-KT Tet-responsive promoter, hKlf4, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-hc-Myc-CMV-KT Tet-responsive promoter, hc-Myc, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-hLin28-CMV-KT Tet-responsive promoter, hLin28, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-SV40LT-CMV-KT Tet-responsive promoter, SV40 LT, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CS-hp53-shRNA-CG Human H1 promoter, hp53-shRNA, CMV promoter, EGFP [PDF] [Word]
For generating iPS cells (Mouse)
Plasmid Name Description Map Sequence Data sheet
CSII-CMV-mOct3/4-IRES2-Venus CMV promoter, mOct3/4, IRES, Venus [PDF] [Word]
CSII-CMV-mSox2-IRES2-Venus CMV promoter, mSox2, IRES, Venus [PDF] [Word]
CSII-CMV-mKlf4-IRES2-Venus CMV promoter, mKlf4, IRES, Venus [PDF] [Word]
CSII-CMV-mc-Myc-IRES2-Venus CMV promoter, mc-Myc, IRES, Venus [PDF] [Word]
CSIV-CMV-mc-Myc-IRES2-Venus CMV promoter, mc-Myc, IRES, Venus, loxP [PDF] [Word]
CSII-EF-mOct3/4-IRES2-Venus EF-1α promoter, mOct3/4, IRES, Venus [PDF] [Word]
CS-UbC-mOct3/4-IRES2-Venus UbC promoter, mOct3/4, IRES, Venus [PDF] [Word]
CSIV-CMV-mOKS-IRES2-Venus CMV promoter, mOct3/4-2A-mKlf4-2A-mSox2, IRES, Venus, loxP [PDF] n.a.
CSIV-TRE-mOct3/4-CMV-KT Tet-responsive promoter, mOct3/4, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-mSox2-CMV-KT Tet-responsive promoter, mSox2, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-mKlf4-CMV-KT Tet-responsive promoter, mKlf4, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CSIV-TRE-mc-Myc-CMV-KT Tet-responsive promoter, mc-Myc, CMV promoter, hKO1-2A-rtTA, loxP [PDF] [Word]
CS-mp53-shRNA-CG Human H1 promoter, mp53-shRNA, CMV promoter, EGFP [PDF] [Word]

Fucci related lentivirus vector – developed by Dr. Miyawaki’s laboratory

Cell cycle indicator Fucci(CA) and Fucci(SA)
Sakaue-Sawano, A. et al., Mol. Cell (2017). doi: 10.1016/j.molcel.2017.10.001.
Plasmid Name Description Map Sequence Data sheet
mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry n.a. n.a. [Data sheet]
mCherry-hCdt1(1/100)Cy(-)/pCSII-EF EF-1α promoter, hCtd1, mCherry n.a. n.a. [Data sheet]
mCherry-hCdt1(1/100)/pCSII-EF EF-1α promoter, hCtd1, mCherry n.a. n.a. [Data sheet]
mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus n.a. n.a. [Data sheet]
tFucci(CA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus n.a. n.a. [Data sheet]
tFucci(CA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus n.a. n.a. [Data sheet]
tFucci(CA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise n.a. n.a. [Data sheet]
tFucci(CA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise n.a. n.a. [Data sheet]
tFucci(SA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus n.a. n.a. [Data sheet]
tFucci(SA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus n.a. n.a. [Data sheet]
tFucci(SA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise n.a. n.a. [Data sheet]
tFucci(SA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise n.a. n.a. [Data sheet]
Cell cycle indicator Fucci2
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).
Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011).
Plasmid Name Description Map Sequence Data sheet
mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry n.a. n.a. [Data sheet]
mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus n.a. n.a. [Data sheet]
Cell cycle indicator
Plasmid Name Description Map Sequence Data sheet
mCherry-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mCherry n.a. n.a. [Data sheet]
mCherry-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mCherry n.a. n.a. [Data sheet]
mVenus-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mVenus n.a. n.a. [Data sheet]